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Related Experiment Videos

Genomic differences between strains of lactate dehydrogenase-elevating virus.

C H Contag, E F Retzel, P G Plagemann

    Intervirology
    |January 1, 1986
    PubMed
    Summary

    Two strains of lactate dehydrogenase-elevating virus (LDV) show minor RNA differences despite distinct antigenic and neurovirulence properties. Most RNA sequences are shared, indicating close genetic relatedness between these LDV variants.

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    Area of Science:

    • Virology
    • Molecular Biology
    • Immunology

    Background:

    • Lactate dehydrogenase-elevating virus (LDV) is an important pathogen in mice.
    • Two LDV strains were isolated exhibiting differences in antigenicity and neurovirulence.

    Purpose of the Study:

    • To compare the RNA of two distinct LDV strains.
    • To investigate the genetic relatedness and physical/biological properties of these LDV strains.

    Main Methods:

    • RNA isolation and T1 RNase fingerprinting.
    • Analysis of virion density, protein molecular weights, and antibody interactions.
    • Replication studies in primary macrophage cultures.

    Main Results:

    • T1 RNase fingerprinting revealed approximately 27 common oligonucleotides and 2-3 unique to each strain.

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  • Both LDV strains were indistinguishable in virion density, protein size, antibody binding, and macrophage replication.
  • LDV RNA demonstrated affinity for oligo(dT), indicating the presence of a poly-A tail.

    • Conclusions:

    • The two LDV strains share a high degree of RNA sequence homology.
    • Despite antigenic and neurovirulence variations, the core genetic makeup of these LDV strains is conserved.
    • The presence of poly-A in LDV RNA was confirmed.