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Updated: Aug 28, 2025
Purification and Analytics of a Monoclonal Antibody from Chinese Hamster Ovary Cells Using an Automated Microbioreactor System
Published on: May 1, 2019
Purification and Analysis of the CREPT Antibody from Mouse Ascites
Zhihao Xie1, Jun Li2, Xinbao Hao1
1Department of Hematology, The First Affiliated Hospital of Hainan Medical University, Haikou 570102, Hainan, China.
Methods:
Cells were cultivated properly to obtain 3E10 CREPT monoclonal antibody cells in the logarithmic growth stage. Monoclonal antibody cells were injected into the abdominal cavity of sensitized mice. The flowing ascites were observed for 7-15 days. The antibody protein was obtained by collection, filtration, dilution, loading, and chromatography. Furthermore, its binding force was detected by SDS-PAGE and Western blot techniques.
Results:
The antibody protein was successfully obtained with a purity of 1895 μg/mL with high liveness.
Conclusion:
This study establishes a one-step purification method for obtaining monoclonal antibody with high liveness and purity for CREPT ascites antibody. This method is simple to perform and lays a foundation for the preparation and purification of humanized monoclonal antibodies in the future. In addition, it provides a basis for further research to investigate how CREPT affects the occurrence and development of different tumors.
