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Updated: Aug 28, 2025

Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens
Published on: March 8, 2018
The complete chloroplast genome of Rubus pacificus (Rosaceae)
Xian-Hua Xiong1, Zheng-Qiao Liao1, Chao Liu1
1College of Life Science and Biotechnology, Mianyang Teachers' College, Mianyang, China.
Abstract:
The complete chloroplast genome of Rubus pacificus, a representative species of R. sect. Malachobatus subsect. Stipulosi, has been characterized by reference-based assembly using Illumina paired-end data. The complete chloroplast genome is 156,255 bp in length, containing a large single-copy region (LSC) of 85,864 bp and a small single-copy region (SSC) of 18,849 bp, which are separated by a pair of inverted repeat regions (IR) of 25,771 bp. A total of 129 genes were predicted from the chloroplast genome, including 85 protein-coding genes, 36 tRNA genes, and eight rRNA genes. According to the present sampling, phylogenetic analysis reveals that R. pacificus and R. laciniatostipulatus form a strongly supported clade and R. sect. Malachobatus subsect. Stipulosi is not monophyletic.
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