Development and Characterization of Flavin-Binding Fluorescent Proteins, Part II: Advanced Characterization
Nora Lisa Bitzenhofer1, Fabienne Hilgers1, Gabriela N Bosio2
1Institute of Molecular Enzyme Technology, Heinrich Heine University Düsseldorf, Forschungszentrum Jülich, Jülich, Germany.
Methods in Molecular Biology (Clifton, N.J.)
|September 15, 2022
Abstract:
Flavin-based fluorescent proteins (FbFPs), a class of small fluorescent proteins derived from light-oxygen-voltage (LOV) domains, bind ubiquitous endogenous flavins as chromophores. Due to their unique properties, they can be used as versatile in vivo reporter proteins under aerobic and anaerobic conditions. This chapter presents methodologies for in-depth characterization of the biochemical, spectroscopic, photophysical, and photochemical properties of FbFPs.


