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Classification of octadecyl-bonded liquid chromatography columns
Journal - Association of Official Analytical Chemists
|May 1, 1987
Summary
This study introduces a classification scheme for octadecyl (C-18) columns based on hydrophobic and silanophilic interactions, aiding in selecting equivalent columns for analytical methods.
Area of Science:
- Analytical Chemistry
- Chromatography
Background:
- Octadecyl (C-18) columns exhibit performance variations, complicating method development and validation.
- Lack of standardized classification hinders reproducible analytical results.
Purpose of the Study:
- To develop a simple classification scheme for C-18 columns.
- To categorize columns based on key retention mechanisms and efficiency.
Main Methods:
- Classification based on hydrophobic interactions (anthracene k:benzene k ratio).
- Assessment of silanophilic interactions (N,N-diethyltoluamide k:anthracene k ratio).
- Inclusion of column efficiency as a third criterion.
Main Results:
- A scheme classifying C-18 columns into 3 major groups based on hydrophobicity, free silanol, and efficiency indices.
- Twelve C-18 packing brands were evaluated using the proposed scheme.
Conclusions:
- The proposed classification scheme aids in selecting similar or equivalent C-18 columns.
- It helps define suitable column types for specific analytical methods, improving reproducibility.