Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA-seq03:21

RNA-seq

10.3K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
10.3K
Next-generation Sequencing03:00

Next-generation Sequencing

92.3K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
92.3K
Sanger Sequencing01:57

Sanger Sequencing

756.3K
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
756.3K
Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

11.4K
In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
11.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Autonomous biomedical research with an artificial intelligence agent.

Science (New York, N.Y.)·2026
Same author

Unified Transcriptome and Mechanics Map of the Intact Mammalian Preimplantation Embryo In Situ.

bioRxiv : the preprint server for biology·2026
Same author

Single-cell eQTL mapping reveals convergent glial-neuronal risk architecture in Parkinson's disease.

bioRxiv : the preprint server for biology·2026
Same author

Disordered protein LAT encodes relative levels of signaling pathways in T cell activation.

Science (New York, N.Y.)·2026
Same author

Multimodal analysis reveals cellular diversity and divergent circuits of the zona incerta.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Concordant transcriptional and morphological remodeling revealed by <i>in vivo</i> Perturb-CLEAR.

bioRxiv : the preprint server for biology·2026

Related Experiment Video

Updated: Aug 28, 2025

Rare Event Detection Using Error-corrected DNA and RNA Sequencing
10:36

Rare Event Detection Using Error-corrected DNA and RNA Sequencing

Published on: August 3, 2018

12.2K

Mostly natural sequencing-by-synthesis for scRNA-seq using Ultima sequencing.

Sean K Simmons1,2, Gila Lithwick-Yanai3, Xian Adiconis1,2

  • 1Klarman Cell Observatory, Broad Institute of MIT and Harvard, Cambridge, MA, USA.

Nature Biotechnology
|September 15, 2022
PubMed
Summary

A new mostly natural sequencing-by-synthesis (mnSBS) method offers cost-effective single-cell RNA sequencing (scRNA-seq). This approach yields results comparable to existing technologies, making it ideal for large-scale projects.

More Related Videos

3' End Sequencing Library Preparation with A-seq2
12:01

3' End Sequencing Library Preparation with A-seq2

Published on: October 10, 2017

10.7K
2D-HELS MS Seq: A General LC-MS-Based Method for Direct and de novo Sequencing of RNA Mixtures with Different Nucleotide Modifications
05:41

2D-HELS MS Seq: A General LC-MS-Based Method for Direct and de novo Sequencing of RNA Mixtures with Different Nucleotide Modifications

Published on: July 10, 2020

2.0K

Related Experiment Videos

Last Updated: Aug 28, 2025

Rare Event Detection Using Error-corrected DNA and RNA Sequencing
10:36

Rare Event Detection Using Error-corrected DNA and RNA Sequencing

Published on: August 3, 2018

12.2K
3' End Sequencing Library Preparation with A-seq2
12:01

3' End Sequencing Library Preparation with A-seq2

Published on: October 10, 2017

10.7K
2D-HELS MS Seq: A General LC-MS-Based Method for Direct and de novo Sequencing of RNA Mixtures with Different Nucleotide Modifications
05:41

2D-HELS MS Seq: A General LC-MS-Based Method for Direct and de novo Sequencing of RNA Mixtures with Different Nucleotide Modifications

Published on: July 10, 2020

2.0K

Area of Science:

  • Genomics
  • Molecular Biology
  • Bioinformatics

Background:

  • Single-cell RNA sequencing (scRNA-seq) is crucial for understanding cellular heterogeneity.
  • Current scRNA-seq methods often involve high costs and complex protocols.
  • Advancements in sequencing technology are needed to improve accessibility and scalability.

Purpose of the Study:

  • To introduce and evaluate a mostly natural sequencing-by-synthesis (mnSBS) method for scRNA-seq.
  • To benchmark mnSBS performance against established scRNA-seq technologies.
  • To assess the utility of mnSBS for diverse scRNA-seq applications and large-scale studies.

Main Methods:

  • Adaptation of a mostly natural sequencing-by-synthesis (mnSBS) approach to the Ultima genomics platform.
  • Utilizing mostly natural, unmodified nucleotides with a low fraction of labeled nucleotides for enhanced polymerase processivity.
  • Application and benchmarking across four distinct scRNA-seq case studies, including 5'/3' scRNA-seq, peripheral blood mononuclear cells (PBMCs), and Perturb-Seq.

Main Results:

  • mnSBS-based scRNA-seq demonstrated high compatibility with current scRNA-seq library preparations.
  • Results from mnSBS closely mirrored those obtained using Illumina sequencing.
  • Minor discrepancies were observed in read positioning relative to gene boundaries due to single-end read characteristics.

Conclusions:

  • The mnSBS method provides a cost-effective and efficient alternative for scRNA-seq.
  • This technology is suitable for various scRNA-seq applications, including multiplexing and Perturb-Seq.
  • mnSBS is poised to facilitate large-scale scRNA-seq projects, enhancing research accessibility.