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Updated: Aug 28, 2025
08:07
Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma
Published on: April 12, 2019
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Circ_0136666 aggravates osteosarcoma development through mediating miR-1244/CEP55 axis.
Xiang Gao1, Nanwei Xu1, Kaisong Miao1
1Department of Orthopedics, Changzhou No.2 People's Hospital, the Affiliated Hospital of Nanjing Medical University, No.29, Xinglong Lane, Tianning District, Changzhou, Jiangsu, China.
Journal of Orthopaedic Surgery and Research
|September 15, 2022
Summary
Circular RNA circ_0136666 promotes osteosarcoma progression by regulating the miR-1244/CEP55 axis. Silencing circ_0136666 offers a potential therapeutic strategy for osteosarcoma.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Circular RNAs (circRNAs) are increasingly recognized for their roles in cancer development.
- The specific function and mechanism of circ_0136666 in osteosarcoma (OS) progression require further investigation.
Purpose of the Study:
- To elucidate the role and underlying mechanism of circ_0136666 in osteosarcoma progression.
Main Methods:
- Quantitative PCR and Western blot for expression analysis.
- Cellular assays (proliferation, migration, invasion, apoptosis, glycolysis) and in vivo xenograft models.
- Dual-luciferase reporter and RNA pull-down assays to confirm molecular interactions.
Main Results:
- Circ_0136666 was upregulated in osteosarcoma tissues and cell lines.
- Circ_0136666 silencing inhibited OS cell proliferation, migration, invasion, and glycolysis, while promoting apoptosis.
- Circ_0136666 sponged microRNA-1244 (miR-1244), leading to increased centrosomal protein 55 (CEP55) expression, thereby promoting OS progression.
Conclusions:
- Circ_0136666 promotes osteosarcoma progression via the circ_0136666/miR-1244/CEP55 pathway.
- Targeting circ_0136666 or CEP55, or restoring miR-1244, presents a potential therapeutic avenue for osteosarcoma.
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