Related Experiment Video
Updated: Aug 28, 2025
A Non-Invasive Method for Generating the Cyclic Loading-Induced Intra-Articular Cartilage Lesion Model of the Rat Knee
Published on: July 5, 2021
[Effect of chronic sleep deprivation on condylar cartilage in rats]
Yue-Hui Bai1, Yang Liu, Yu-Lan Cui
1Department of Prosthodontics, School and Hospital of Stomatology, Hebei Medical University. Shijiazhuang 050017, China.
Purpose:
The aim of this study was to investigate the morphological changes of condylar cartilage of temporomandibular joint (TMJ) and the expression changes of IL-1β,TNF-α,IGF-1 and VEGF in condylar cartilage of TMJ by establishing a chronic sleep deprivation model in rats.
Methods:
Sixty rats were randomly divided into experimental group, control group and recovery group. Modified multiple platforms method (MMPM) was used to build chronic sleep deprivation models in experimental and recovery groups. Rats in the recovery group received 1 week of cage feeding after sleep deprivation. H-E staining was used to observe morphological change of the condyle. Immunohistochemical method was performed to detect the changes of IL-1β, TNF-α, IGF-1 and VEGF. The data was processed by using SPSS 23.0 software package.
Results:
MMPM can establish chronic sleep deprivation model effectively. H-E staining showed condylar cartilage of the experimental group was split stripped, and the boundaries of cartilage cell layer became blurred. Compared with the control group, the recovery group had less cracks in the fibrous layer or some of the cracks were occupied by fibrous tissue. Immunohistochemistry showed that the positive expression intensity of IL-1β and TNF-α in the experimental group was significantly higher than in the control group (P＜0.05), the positive expression intensity in the recovery group was significantly lower than in the experimental group(P＜0.05). The positive expression intensity of IGF-1 and VEGF in the experimental group was significantly higher than in the control group(P＜0.05). The expression of IGF-1 and VEGF decreased significantly in the recovery group which received sleep deprivation no more than 3 weeks(P＜0.05).
Conclusions:
Chronic sleep deprivation can increase the expression of IL-1β, TNF-α and VEGF in condylar cartilage and aggravate osteoarthritis. Chronic sleep deprivation can lead to increase of IGF-1 in condylar cartilage tissue, which plays a crucial role in protecting and promoting the reconstruction of condylar cartilage. After chronic sleep deprivation, the expressions of IL-1β, TNF-α, IGF-1 and VEGF in the condylar cartilage of rats were decreased after 1 week of recovery, and the condylar cartilage underwent restorative reconstruction.
