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Genetic Manipulation of the Mouse Developing Hypothalamus through In utero Electroporation

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Published on: July 24, 2013

Epigenetic manipulation to improve mouse SCNT embryonic development.

Yamei Li^1,2,3, Qiang Sun^1,2,3

¹University of Chinese Academy of Sciences, Beijing, China.

Frontiers in Genetics

|September 16, 2022

Summary

This summary is machine-generated.

Somatic cell nuclear transfer (SCNT) cloning faces low efficiency due to incomplete epigenetic reprogramming in cloned embryos. This review explores epigenetic barriers and strategies to improve cloned animal development and reduce abnormalities.

Keywords:

cloning efficiency epigenetic barriers post-implantation pre-implantation somatic cell nuclear transfer

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Published on: November 29, 2012

Area of Science:

Reproductive biology
Epigenetics
Developmental biology

Background:

Somatic cell nuclear transfer (SCNT) is a technique for cloning mammals.
Low efficiency of SCNT is a major limitation for its application.
Cloned embryos often exhibit developmental abnormalities due to incomplete epigenetic reprogramming.

Purpose of the Study:

To review recent epigenetic barriers encountered in SCNT embryos.
To discuss strategies for correcting epigenetic defects in SCNT.
To improve the developmental potential and reduce abnormalities in cloned animals.

Main Methods:

Review of current literature on SCNT and epigenetic reprogramming.
Analysis of epigenetic modifications in cloned embryos.
Evaluation of novel strategies for epigenetic correction.

Main Results:

Incomplete reprogramming of somatic cell epigenetics is a key barrier to SCNT efficiency.
Specific epigenetic marks (e.g., DNA methylation, histone modifications) are often improperly reset.
Various strategies, including chemical treatments and genetic manipulations, are being explored to enhance reprogramming.

Conclusions:

Addressing epigenetic barriers is crucial for improving SCNT efficiency and developmental outcomes.
Successful epigenetic reprogramming is essential for producing healthy cloned animals.
Further research into epigenetic regulation in SCNT holds promise for advancing reproductive technologies.