Biodegradation modeling of phenol using Curtobacterium flaccumfaciens as plant-growth-promoting bacteria

Phenol is a major worry pollutant resulting from industrialized manufacturing and chemical reactions. The growth kinetics and biodegradation of phenol were initially investigated using C. flaccumfaciens, a recently identified plant growth stimulating bacterium. Based on the Haldane inhibition model, Haldane's growth kinetics inhibition coefficient (Ki), half-saturation coefficient (Ks), and the maximum specific growth rate (max) for phenol-dependent growth kinetics were estimated to be 329 (mg/L), 9.14 (mg/L), and 1.05 (h^-1), respectively. With a sum of squared error (SSR) of 1.36 × 10^-3, the Haldane equation is well adapted to empirical data. The improved Gombertz model also accurately predicts phenol biodegradation trends. The rate of phenol biodegradation and the lag time both increased as the initial phenol concentrations were increased. C. flaccumfaciens growth and phenol biodegradation were best achieved at a pH of 7.0 at a temperature of 28 °C incubation. A phenol biodegradation mechanism by C. flaccumfaciens has been proposed. In conclusion, this study revealed the ability of C. flaccumfaciens to promote plant growth and biodegrade phenol simultaneously. This could aid in rhizoremediation and crop yield preservation in phenol-stressed conditions.