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A sandwich ELISA for bovine serum in viral vaccines
Journal of Biological Standardization
|July 1, 1987
Abstract:
A double antibody sandwich ELISA for the detection of bovine serum in viral vaccines was developed and standardized with commercially available reagents. The detection limit by ELISA was 0.5 ng ml-1. ELISA was found to be 50-400 times more sensitive than the currently used assays. It was concluded that ELISA is a specific, sensitive and reproducible method for the determination of residual amounts of bovine serum in viral vaccines.