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Reconstructing electron transfer components from an Fe(II) oxidizing bacterium.

Abhiney Jain1, Madison J Kalb1, Jeffrey A Gralnick1

  • 1BioTechnology Institute and Department of Plant and Microbial Biology, University of Minnesota - Twin Cities, St. Paul, MN 55108, USA.

Microbiology (Reading, England)
|September 16, 2022
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Summary

Neutrophilic iron-oxidizing bacteria use extracellular electron transfer (EET) for metabolism. This study validated components of two EET pathways in *Sideroxydans lithotrophicus* ES-1, revealing complex electron transfer mechanisms.

Keywords:
Extracellular electron transferMtoSideroxydansporin-periplasmicc-type cytochrome complex

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Area of Science:

  • Microbiology
  • Biogeochemistry
  • Bioenergetics

Background:

  • Neutrophilic iron-oxidizing bacteria are crucial for biogeochemical cycles and technological applications.
  • These microbes are believed to perform extracellular electron transfer (EET) to oxidize Fe(II) externally.
  • *Sideroxydans lithotrophicus* ES-1 is a freshwater bacterium with predicted EET pathways, but it is difficult to culture and not genetically tractable.

Purpose of the Study:

  • To investigate the function of predicted extracellular electron transfer (EET) pathways in *Sideroxydans lithotrophicus* ES-1.
  • To validate the roles of specific inner membrane and periplasmic protein components in Fe(II) oxidation.
  • To explore the potential complexity of EET mechanisms in this organism.

Main Methods:

  • Expressed components of the Mto and Slit_0867-0870 PCC3 EET pathways from *S. lithotrophicus* ES-1 into *Aeromonas hydrophila*.
  • Utilized *Aeromonas hydrophila* as a model organism for studying EET.
  • Assessed the complementation of EET activity in *Aeromonas* mutants lacking native pathway components.

Main Results:

  • Combinations of putative inner membrane and periplasmic components from the Mto and Slit_0867-0870 PCC3 pathways partially restored EET activity in *Aeromonas* mutants.
  • Provided evidence for the electron transfer functionality of these components.
  • Demonstrated interactions between inner membrane and periplasmic elements of the Mto and Slit_0867-0870 PCC3 pathways.

Conclusions:

  • The study provides functional evidence for components of two proposed EET pathways in *S. lithotrophicus* ES-1.
  • Suggests that EET in *S. lithotrophicus* ES-1 may be more complex than previously understood.
  • Raises questions about the directionality and intricate mechanisms of these electron transfer pathways.