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Inhibitory mechanism of ternary fusion protein MSIK on HPV virus
1Affiliated Hospital, Hunan Polytechnic of Environment and Biology, Hengyang, China. huhengfenfen@126.com.
Objective:
We investigated whether the ternary fusion protein (MBP-SERPINA3-IFN-κ, MSIK) can inhibit the proliferation of human papillomavirus (HPV) and the possible mechanism of this inhibitory effect.
Materials And Methods:
First, the purchased MSIK protein was prepared into MSIK protein solutions of different concentrations, and then epithelial cells and 300 mice were involved in the research. The HSV-1 virus was used as the infection pathogen to explore the mechanism of MSIK reinhibition of HPV virus. The virus content in each sample was detected by PCR technology.
Results:
Epithelial cells treated with different concentrations of MSIK had inhibitory effects on the invasion and replication of HSV-1 virus. MSIK protein had a positive effect on wound healing in mice at the same time, and it had an inhibitory effect on the invasion of HSV-1 virus, and the higher concentration of MSIK, the better the effect.
Conclusions:
MSIK can quickly help wound healing and inhibit the replication of HSV-1 virus. It can also effectively prevent the influx of HPV virus and replication has a positive effect on the prevention and treatment of HPV virus.
Insights
The novel ternary fusion protein, MBP-SERPINA3-IFN-κ (MSIK), demonstrates significant potential in inhibiting human papillomavirus (HPV) replication and promoting wound healing. Further research confirms its efficacy in preventing viral influx and replication.
Area of Science:
- Virology
- Immunology
- Biochemistry
Background:
- Human papillomavirus (HPV) infections are a significant global health concern.
- Current treatments for HPV infections have limitations, necessitating the development of novel therapeutic agents.
- The ternary fusion protein MBP-SERPINA3-IFN-κ (MSIK) has been engineered for potential antiviral applications.
Purpose of the Study:
- To investigate the efficacy of MSIK in inhibiting HPV proliferation.
- To elucidate the underlying mechanisms of MSIK's antiviral activity against HPV.
- To evaluate MSIK's impact on wound healing in a preclinical model.
Main Methods:
- MSIK protein was prepared in various concentrations.
- Epithelial cells and 300 mice were utilized in the study.
- Herpes simplex virus type 1 (HSV-1) was used to model viral infection, and viral content was quantified using PCR.
Main Results:
- MSIK demonstrated dose-dependent inhibition of HSV-1 invasion and replication in epithelial cells.
- MSIK significantly accelerated wound healing in mice.
- Higher concentrations of MSIK correlated with enhanced inhibition of HSV-1 invasion.
Conclusions:
- MSIK exhibits potent antiviral properties, effectively inhibiting viral replication and preventing viral influx.
- MSIK demonstrates a dual therapeutic effect, promoting wound healing while combating viral infections.
- MSIK shows promise as a therapeutic agent for the prevention and treatment of HPV infections.
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