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Author Spotlight: A Simple and Cost-Effective Method for Leukocyte Analysis in Semen
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Standards in semen examination: publishing reproducible and reliable data based on high-quality methodology
Lars Björndahl1, Christopher L R Barratt2, David Mortimer3
1ANOVA, Karolinska University Hospital and Karolinska Institutet, Stockholm, Sweden.
Human Reproduction (Oxford, England)
|September 16, 2022
Summary
New guidelines enhance transparency in biomedical research, particularly for semen examination studies. Adhering to updated WHO and ISO standards, along with checklists, is recommended for publication in peer-reviewed journals.
Area of Science:
- Biomedical Science
- Reproductive Health
- Laboratory Medicine
Background:
- Biomedical science increasingly emphasizes transparency, openness, and reproducibility.
- Studies relying on basic semen examination require rigorous standards for reliable results.
Purpose of the Study:
- To propose standardized methods for publishing studies based on semen examination.
- To advocate for the adoption of updated WHO and ISO guidelines in scientific publications.
Main Methods:
- Highlighting the recent publication of the 6th edition of the WHO Laboratory Manual for the Examination and Processing of Human Semen.
- Referencing the International Standard ISO 23162:2021 as a key resource.
- Suggesting the use of a checklist to ensure adherence to laboratory methods.
Main Results:
- Two concordant documents (WHO Manual and ISO Standard) provide updated methodologies.
- These tools offer a framework for improving the quality and consistency of semen examination studies.
Conclusions:
- Authors should be instructed to follow the latest WHO and ISO laboratory methods for semen examination.
- Implementing these standards, possibly via a checklist, will enhance the reproducibility and credibility of published research.

