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Key methodological challenges in detecting circulating miRNAs in different biofluids.

Chitra Mestry1, Tester F Ashavaid2, Swarup Av Shah2

  • 1Research Laboratories, 29537P. D. Hinduja Hospital & Medical Research Centre, Mahim, India.

Annals of Clinical Biochemistry
|September 16, 2022
PubMed
Summary
This summary is machine-generated.

Circulating microRNAs (miRNAs) show promise as disease biomarkers. This review highlights factors affecting their detection, crucial for accurate disease risk prediction and improved patient outcomes.

Keywords:
Circulating miRNAbiofluidsbiomarkerepigeneticspreanalytical factor

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Genomics

Background:

  • Technological advances improve disease prediction, but novel biomarkers are needed to reduce morbidity and mortality.
  • Circulating microRNAs (miRNAs) are key regulators in multifactorial diseases and are emerging biomarkers due to accessibility and stability.

Purpose of the Study:

  • To review pre-analytical and analytical factors influencing circulating miRNA detection.
  • To address the lack of consensus on standardized methods for miRNA analysis.

Main Methods:

  • Literature review summarizing studies on circulating miRNA stability, processing, and quantification.
  • Analysis of controllable and uncontrollable factors affecting miRNA expression profiles.

Main Results:

  • Pre-analytical factors like sample selection and methodological aspects significantly impact circulating miRNA detection.
  • Inconsistent methodologies lead to erroneous miRNA expression profiles.

Conclusions:

  • Standardization of pre-analytical and analytical procedures is essential for reliable circulating miRNA biomarker development.
  • Addressing these factors will enhance the accuracy of disease risk prediction using circulating miRNAs.