Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

13.5K
The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...
13.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Machine Learning Approach for Enumeration of Circulating Cells with Diffuse <i>in vivo</i> Flow Cytometry.

bioRxiv : the preprint server for biology·2026
Same author

Considerations for the use of targeted fluorescence contrast agents to detect circulating cancer cell populations with diffuse <i>in vivo</i> flow cytometry.

Journal of biomedical optics·2026
Same author

Engineering NIR Probes to Enhance Affinity and Clinical Workflow Compatibility for Prostate Cancer Imaging.

Angewandte Chemie (International ed. in English)·2025
Same author

Engineering NIR probes to enhance affinity and clinical workflow compatibility for prostate cancer imaging.

bioRxiv : the preprint server for biology·2025
Same author

Considerations for the use of contrast agents with diffuse <i>in vivo</i> flow cytometry to detect circulating cancer cell populations.

bioRxiv : the preprint server for biology·2025
Same author

Noncontact Fiber Optic Probe for Clinical Applications of Raman Spectroscopy.

Applied spectroscopy·2025

Related Experiment Video

Updated: Aug 28, 2025

Validation of Nanobody and Antibody Based In Vivo Tumor Xenograft NIRF-imaging Experiments in Mice Using Ex Vivo Flow Cytometry and Microscopy
08:09

Validation of Nanobody and Antibody Based In Vivo Tumor Xenograft NIRF-imaging Experiments in Mice Using Ex Vivo Flow Cytometry and Microscopy

Published on: April 6, 2015

11.7K

Near-infrared diffuse in vivo flow cytometry.

Joshua Pace1, Fernando Ivich1, Eric Marple2

  • 1Northeastern University, Department of Bioengineering, Boston, Massachusetts, United States, United States.

Journal of Biomedical Optics
|September 17, 2022
PubMed
Summary

Near-infrared diffuse in vivo flow cytometry (NIR-DiFC) enables noninvasive detection of circulating tumor cells (CTCs) in mice. This advancement offers lower autofluorescence and paves the way for human applications.

Keywords:
contrast agentsdiffuse fluorescencediffuse in vivo flow cytometrynear-infrared light

More Related Videos

Analysis of T-cell Receptor-Induced Calcium Influx in Primary Murine T-cells by Full Spectrum Flow Cytometry
10:01

Analysis of T-cell Receptor-Induced Calcium Influx in Primary Murine T-cells by Full Spectrum Flow Cytometry

Published on: December 16, 2022

4.2K
A Bright NIR-II Fluorescence Probe for Vascular and Tumor Imaging
05:51

A Bright NIR-II Fluorescence Probe for Vascular and Tumor Imaging

Published on: March 17, 2023

1.9K

Related Experiment Videos

Last Updated: Aug 28, 2025

Validation of Nanobody and Antibody Based In Vivo Tumor Xenograft NIRF-imaging Experiments in Mice Using Ex Vivo Flow Cytometry and Microscopy
08:09

Validation of Nanobody and Antibody Based In Vivo Tumor Xenograft NIRF-imaging Experiments in Mice Using Ex Vivo Flow Cytometry and Microscopy

Published on: April 6, 2015

11.7K
Analysis of T-cell Receptor-Induced Calcium Influx in Primary Murine T-cells by Full Spectrum Flow Cytometry
10:01

Analysis of T-cell Receptor-Induced Calcium Influx in Primary Murine T-cells by Full Spectrum Flow Cytometry

Published on: December 16, 2022

4.2K
A Bright NIR-II Fluorescence Probe for Vascular and Tumor Imaging
05:51

A Bright NIR-II Fluorescence Probe for Vascular and Tumor Imaging

Published on: March 17, 2023

1.9K

Area of Science:

  • Biomedical Optics
  • Flow Cytometry
  • In Vivo Imaging

Background:

  • Diffuse in vivo flow cytometry (DiFC) is an emerging noninvasive technique for detecting circulating cells.
  • Previous DiFC versions used red and blue-green light.
  • Near-infrared (NIR) light offers deeper tissue penetration and compatibility with NIR contrast agents.

Purpose of the Study:

  • To design and demonstrate a NIR-DiFC instrument.
  • To evaluate its performance in vitro and in vivo.
  • To enable detection of rare cells using NIR fluorescence.

Main Methods:

  • Developed an improved optical fiber probe for efficient fluorescence collection and autofluorescence rejection.
  • Constructed a NIR-DiFC instrument.
  • Tested the system with NIR fluorescent microspheres, OTL38-labeled cell lines in flow phantoms, and OTL38-labeled L1210A cells in nude mice.

Main Results:

  • NIR-DiFC detected circulating tumor cells (CTCs) in flow phantoms with high signal-to-noise ratios (SNRs) of 19-32 dB.
  • In vivo, fluorescently labeled CTCs were detected in mice with a mean SNR of 26 dB.
  • NIR-DiFC demonstrated significantly lower autofluorescence and false-alarm rates compared to blue-green DiFC.

Conclusions:

  • NIR-DiFC enables the use of emerging NIR contrast agents for cell detection.
  • This technique allows for noninvasive enumeration of circulating cells in deeper tissues.
  • The developed NIR-DiFC system holds promise for future clinical applications in humans.