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In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice
Published on: August 23, 2019
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Microarray profiling identifies hsa_circ_0082003 as a novel tumor promoter for papillary thyroid carcinoma
1Department of Thyroid Surgery, The Third Affiliated Hospital of Soochow University, Changzhou First People's Hospital, Changzhou, Jiangsu, China.
Journal of Endocrinological Investigation
|September 17, 2022
Summary
Circular RNAs (circRNAs) play a role in papillary thyroid carcinoma (PTC) progression. Hsa_circ_0082003 is upregulated in PTC and may serve as a diagnostic biomarker and therapeutic target.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Circular RNAs (circRNAs) are non-coding RNAs implicated in tumor development.
- This study investigates the role of circRNAs in papillary thyroid carcinoma (PTC) progression.
Purpose of the Study:
- To explore the involvement of circRNAs in PTC development.
- To identify specific circRNAs as potential diagnostic biomarkers and therapeutic targets for PTC.
Main Methods:
- circRNA microarray analysis to identify differentially expressed circRNAs (DECs) in PTC tissues.
- Bioinformatic analyses to predict the functions of DECs.
- RT-qPCR to validate hsa_circ_0082003 expression and its correlation with clinicopathological parameters.
- In vitro assays (cell counting kit-8, colony formation, wound healing, Transwell) to assess the biological functions of hsa_circ_0082003 in PTC cells.
- In vivo tumor formation assays in nude mice to validate the role of hsa_circ_0082003 in PTC tumorigenesis.
Main Results:
- 3150 DECs were identified, with 2317 upregulated and 833 downregulated.
- Pathway enrichment analyses suggested roles for DECs in PTC development.
- Hsa_circ_0082003 was significantly upregulated in PTC tissues and correlated with poor clinicopathological parameters.
- Hsa_circ_0082003 demonstrated good diagnostic performance for PTC and lymph node metastasis detection.
- Downregulation of hsa_circ_0082003 inhibited PTC cell proliferation, migration, invasion, and tumor growth in vivo.
Conclusions:
- Hsa_circ_0082003 is significantly upregulated in papillary thyroid carcinoma.
- Hsa_circ_0082003 shows potential as a diagnostic biomarker for PTC and lymph node metastasis.
- Hsa_circ_0082003 may represent a novel therapeutic target for PTC treatment.
Keywords:
BiomarkerCircRNA microarrayCircular RNAsHsa_circ_0082003Papillary thyroid carcinomaTumor promoterMore Related Videos
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