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Sensitive and selective determination of vitamin B2 in non-alcoholic beverage and milk samples at poly (glutamic
Gizaw Tesfaye1, Negussie Negash2, Merid Tessema2
1Department of Chemistry, College of Natural and Computational Sciences, Addis Ababa University, P. O. Box 1176, Addis Ababa, Ethiopia. gizawtes@gmail.com.
Background:
The deficiency of vitamin B2 can lead to many health problems. Therefore, it is necessary to develop a sensitive, selective and fast method for the determination of vitamin B2 in food samples. In this work, a sensitive, selective and low-cost electrochemical sensor was developed using poly (glutamic acid) and Zinc oxide nanoparticles (ZnO NPs) for vitamin B2 in non-alcoholic beverage and milk samples.
Methods:
The modification of the electrode surface was carried out by electropolymerization of glutamic acid on ZnO NPs-carbon paste electrode (ZnO NPS-CPE). The prepared electrodes were characterized by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), scanning electron microscopy (SEM) and X-Ray diffraction (XRD). CV and square wave voltammetry (SWV) were used to investigate the electrochemical behavior of vitamin B2 at the modified electrode. The effect of various parameters such as amount of ZnO NPs, polymerization cycle, concentration of the monomer, pH, scan rate and accumulation time were optimized to obtain maximum sensitivity at the modified electrode.
Results:
The developed sensor showed high electrocatalytic activity towards vitamin B2. Under the optimized conditions, the developed sensor showed a linear response in the range 0.005-10 µM with a low detection limit of (LOD) 0.0007 ± 0.00001 µM and high sensitivity of 21.53 µA/µM.
Conclusions:
A reproducible, repeatable, stable and selective sensor was successfully applied for the quantification of vitamin B2 in beverage and milk samples with acceptable recoveries in the range of 88-101%.
