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Published on: February 21, 2019
Proteomics dataset from 26th Dynasty Egyptian mummified remains sampled using minimally invasive skin sampling tape
Dylan H Multari1, Prathiba Ravishankar1, Geraldine J Sullivan1
1School of Natural Sciences, Macquarie University, North Ryde, NSW 2109, Australia.
Abstract:
Paleoproteomics typically involves the destructive sampling of precious bioarchaeological materials. This analysis aims to investigate the proteins identifiable via nanoLC-MS/MS from highly degraded 26th Dynasty Egyptian mummified human remains (NMR.29.1-8) after non-destructive sampling with commercially available dermatology-grade skin sampling tape strips. A collection of cranial and other bone fragments were sampled with the tape strips then subsequently analysed using a shotgun proteomics approach. The number of proteins identified using this method ranged from 18 to 437 at a peptide FDR of <1%. Deamidation ratios were assessed using an in-house R script, with asparagine deamidation averaging ∼20-30% and glutamine deamidation averaging ∼15-25%.
