Related Experiment Video

Updated: Aug 28, 2025

Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome
07:23

Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome

Published on: June 15, 2016

8.6K

MicroRNA-1306-5p Regulates the METTL14-Guided m6A Methylation to Repress Acute Myeloid Leukemia

Jiajia Li1, Yanping Wu1, Meng Wang1

  • 1Department of Hematology, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui, China.

Computational and Mathematical Methods in Medicine
|September 19, 2022
PubMed

Insights

MicroRNAs (miRNAs) and m6A methylation are crucial in cancer. In acute myeloid leukemia (AML), suppressed miR-1306-5p promotes cancer progression by increasing m6A methylation via METTL14 upregulation.

Area of Science:

  • Oncology
  • Molecular Biology
  • Epigenetics

Background:

  • MicroRNAs (miRNAs) and N6-methyladenosine (m6A) methylation are key regulators in cancer.
  • The precise relationship between miRNA and m6A methylation in acute myeloid leukemia (AML) remains incompletely understood.

Purpose of the Study:

  • To elucidate the effect of m6A methylation induced by miRNAs on AML.
  • To investigate the underlying molecular mechanisms connecting miRNA, m6A methylation, and AML progression.

Main Methods:

  • Quantitative real-time PCR (qRT-PCR) and Western blot to assess METTL14 expression.
  • Cell proliferation assays (CCK-8, Transwell, flow cytometry) and in vivo tumor models with Ki-67 staining.
  • Dual-luciferase reporter assays, dot blot analysis for m6A methylation detection.

Main Results:

  • METTL14 was found to be upregulated in AML patients, and its overexpression promoted AML development.
  • miR-1306-5p was identified as a direct targeting miRNA of METTL14; its overexpression alleviated AML progression.
  • The m6A methylation level, regulated by METTL14, was shown to be influenced by miR-1306-5p.

Conclusions:

  • Suppressed miR-1306-5p enhances AML progression by increasing m6A methylation levels through METTL14 upregulation.
  • These findings offer a mechanistic basis for developing novel therapeutic strategies for AML.

More Related Videos

Antibody-Free Assay for RNA Methyltransferase Activity Analysis08:31

Antibody-Free Assay for RNA Methyltransferase Activity Analysis

Published on: July 9, 2019

7.3K
A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues08:56

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues

Published on: December 5, 2016

11.0K

Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.1K
Master Transcription Regulators02:23

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
7.0K
Epigenetic Regulation01:46

Epigenetic Regulation

Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
31.3K
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
983
Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
7.1K
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
23.0K