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Integrative analysis of synovial sarcoma transcriptome reveals different types of transcriptomic changes
Zhengwang Sun1, Mengchen Yin2, Yi Ding3
1Department of Musculoskeletal Oncology, Fudan University Shanghai Cancer Center, Shanghai, China.
Frontiers in Genetics
|September 19, 2022
Summary
This study reveals significant transcriptomic alterations in synovial sarcoma (SS), including gene expression changes, alternative splicing, and novel gene fusions. These findings offer new insights into SS biology and molecular mechanisms.
Area of Science:
- Oncology
- Genomics
- Molecular Biology
Background:
- Synovial sarcoma (SS) is a rare, aggressive soft tissue cancer.
- The comprehensive transcriptomic landscape of SS remains poorly understood.
Purpose of the Study:
- To systematically dissect the transcriptomic profile of SS.
- To identify changes in gene expression, alternative splicing, gene fusions, and circular RNAs in SS.
Main Methods:
- Deep total RNA sequencing was performed on ten paired SS and tumor-adjacent tissues.
- Analysis included gene expression, alternative splicing, gene fusion, and circular RNA profiling.
Main Results:
- Identified 2,309 upregulated and 1,977 downregulated genes, impacting cell cycle and metabolic pathways.
- Detected differential alternative splicing in 2,511 genes, suggesting its role in tumorigenesis.
- Discovered known and 11 novel gene fusions, along with 49 differentially expressed circular RNAs.
Conclusions:
- Comprehensive transcriptomic analysis provides novel insights into SS biology.
- Both transcriptional and post-transcriptional modifications contribute to SS development.
- Findings advance understanding of the molecular mechanisms underlying synovial sarcoma.
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