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Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein
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Paraoxonase 1 gene polymorphisms in lipid oxidation and atherosclerosis development
Marija Vavlukis1,2, Ana Vavlukis3, Katerina Krsteva3
1University Clinic for Cardiology, Skopje, North Macedonia.
Frontiers in Genetics
|September 19, 2022
Summary
Paraoxonase 1 (PON1) gene variations influence cardiovascular disease risk by affecting its antioxidant and lipid-modifying functions. PON1 genotyping may benefit high-risk cardiovascular patients.
Area of Science:
- Biochemistry
- Genetics
- Cardiology
Background:
- Paraoxonase 1 (PON1) is a calcium-dependent enzyme with antioxidant, anti-inflammatory, and lipid-modifying properties.
- Clinical studies link PON1 gene polymorphisms to various cardiovascular pathologies.
- PON1 exhibits aryldialkylphosphatase, arylesterase, and lactonase activities.
Purpose of the Study:
- To explore the association between PON1 gene polymorphisms and cardiovascular risk.
- To investigate the impact of specific PON1 SNPs on enzyme activity and serum concentrations.
- To determine whether PON1 genotype or phenotype is primarily associated with cardiovascular risk.
Main Methods:
- Review of clinical studies on PON1 polymorphisms and cardiovascular diseases.
- Identification of commonly studied PON1 SNPs in coding (rs622, rs854560) and regulatory (rs705381, rs854572, rs705379) regions.
- Analysis of the effects of SNPs on PON1 enzyme activities and serum levels.
Main Results:
- Different PON1 polymorphisms are associated with varying cardiovascular risks.
- Specific SNPs influence PON1 serum concentrations and enzyme activities, leading to pro- or anti-atherogenic effects.
- The interplay between PON1 genotype and phenotype in cardiovascular risk remains complex.
Conclusions:
- PON1 gene variations play a significant role in cardiovascular health.
- Understanding PON1 SNP effects on enzyme function is crucial for assessing cardiovascular risk.
- PON1 genotyping may be a valuable tool for managing patients with high cardiovascular risk.
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