Transcriptome analysis of tea (Camellia sinensis) leaves in response to ammonium starvation and recovery

The tea plant is a kind of ammonium-preferring crop, but the mechanism whereby ammonium (NH₄ ⁺) regulate its growth is not well understood. The current study focused on the effects of NH₄ ⁺ on tea plants. Transcriptomic analysis was performed to investigate the early- and late-stage NH₄ ⁺ deprivation and resupply in tea plants shoots. Through short- and long-term NH₄ ⁺ deficiency, the dynamic response to NH₄ ⁺ stress was investigated. The most significant effects of NH₄ ⁺ deficiency were found to be on photosynthesis and gene ontology (GO) enrichment varied with the length of NH₄ ⁺ deprivation. Enriched KEGG pathways were also different when NH₄ ⁺ was resupplied at different concentrations which may indicate reasons for tolerance of high NH₄ ⁺ concentration. Using weighted gene co-expression network analysis (WGCNA), modules related to significant tea components, tea polyphenols and free amino acids, were identified. Hence, NH₄ ⁺ could be regarded as a signaling molecule with the response of catechins shown to be higher than that of amino acids. The current work represents a comprehensive transcriptomic analysis of plant responses to NH₄ ⁺ and reveals many potential genes regulated by NH₄ ⁺ in tea plants. Such findings may lead to improvements in nitrogen efficiency of tea plants.