Related Experiment Video
Updated: Aug 28, 2025

Curtain Flow Column: Optimization of Efficiency and Sensitivity
Published on: June 12, 2016
Numerical Approximation of the Nonequilibrium Model of Gradient Elution Chromatography Considering Linear and
1Department of Mathematics, COMSATS University, Park Road, Chak Shahzad, Islamabad 45550, Pakistan.
Abstract:
In both linear and nonlinear chromatography, the lumped kinetic model is a suitable model for predicting elution bands when appropriate equilibrium functions and mass transfer coefficients are accessible. This model also works well in the case of gradient elution chromatography if variations in the equilibrium functions due to changes in the mobile phase composition are known. The rational selection of an optimum gradient is explored in this study from three different perspectives using the lumped kinetic model. Elution profiles generated by using (a) linear solvent strength, (b) quadratic solvent strength, and (c) power law are investigated. The effectiveness and reliability of the suggested numerical approach, utilizing the flux-limiting finite volume method, are demonstrated through numerical simulations. The impacts of axial dispersion, nonlinearity coefficient, Henry's constant, mass transfer coefficient, and gradient parameters are studied on single and two-component elution profiles.
Related Concept Videos
Column Efficiency: Rate Theory
During elution, a solute molecule experiences numerous transitions between stationary and mobile phases, exhibiting irregular residence times in...
High-Performance Liquid Chromatography: Elution Process
Optimizing Chromatographic Separations
Band broadening refers to spreading solute bands as they travel through the column. This broadening can impact resolution. Plate height (H) represents the length required for one theoretical plate. A lower plate height corresponds to...
Analyte Adsorption and Distribution
Mechanistic Models: Compartment Models in Algorithms for Numerical Problem Solving
In individual population analyses, different algorithms are employed, such as Cauchy's method, which uses a...
Linear Approximation in Time Domain
For a simple pendulum with a mass evenly distributed along its length and the center of mass located at half the pendulum's length,...

