Simple neutralization test report: Do probiotics contribute to COVID-19 therapy?

Area of Science:

• Immunology
• Microbiology
• Virology

Background:

• The urgent need for effective COVID-19 therapies has increased interest in supplements.
• Probiotic consumption is a popular strategy to enhance the host immune system.

Purpose of the Study:

• To investigate the effect of antibodies from probiotic consumers on COVID-19 cell models.
• To assess the influence on lactate dehydrogenase (LDH), adenosine triphosphate (ATP) levels, and cell viability.

Main Methods:

• An experimental study involving control and intervention groups with varying antibody levels and probiotic intake.
• In vitro assays on peripheral blood mononuclear cells (PBMCs) exposed to SARS-CoV-2 spike protein.
• Cytotoxicity assessment using LDH, ATP, and XTT assays.

Main Results:

• Significant differences in LDH and ATP values were observed across groups (p < 0.001).
• A trend of decreased cell viability (XTT assay) correlated with increased LDH and ATP levels.
• Probiotic non-consumers showed the most significant LDH changes, while probiotic consumers with specific antibody levels exhibited higher cytopathic activity.

Conclusions:

• LDH and ATP levels significantly influence cell viability in COVID-19 models.
• Antibodies from individuals consuming probiotics may modulate cellular responses to SARS-CoV-2 infection.
• Probiotic intake could play a role in the immune response against COVID-19.