Related Experiment Video
Updated: Aug 28, 2025

In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity
Published on: March 25, 2020
If It Is Hard, It Is Worth Doing: Engineering Radical Enzymes from Anaerobes
Christof M Jäger1, Anna K Croft1
1Sustainable Process Technologies Group, University of Nottingham, Nottingham NG7 2RD, United Kingdom.
Abstract:
With a pressing need for sustainable chemistries, radical enzymes from anaerobes offer a shortcut for many chemical transformations and deliver highly sought-after functionalizations such as late-stage C-H functionalization, C-C bond formation, and carbon-skeleton rearrangements, among others. The challenges in handling these oxygen-sensitive enzymes are reflected in their limited industrial exploitation, despite what they may deliver. With an influx of structures and mechanistic understanding, the scope for designed radical enzymes to deliver wanted processes becomes ever closer. Combined with new advances in computational methods and workflows for these complex systems, the outlook for an increased use of radical enzymes in future processes is exciting.
Related Concept Videos
Radical Reactivity: Electrophilic Radicals
Restriction Enzymes
The host bacteria protect their own genomic DNA from these enzymes by methylating these sites. Some...
Overview of Archaea
Diversity of Archaea I
Radical Reactivity: Nucleophilic Radicals
Radical Formation: Elimination

