Morphology of physiologically identified retinogeniculate X- and Y-axons in the cat

1. We studied the morphology of individual, physiologically identified retinogeniculate axons in normal adult cats. The axons were recorded in the lateral geniculate nucleus or in the subjacent optic tract, characterized as X or Y by physiological criteria, penetrated, and injected with horseradish peroxidase. With subsequent application of appropriate histochemistry, the enzyme provides a complete label of the terminal arbors and parent trunks for morphological analysis. We have recovered for such analysis 26 X- and 25 Y-axons; of these, 14 X- and 12 Y-axons were studied in detail. 2. Within the optic tract, the parent trunk of every X-axon is located closer to the lateral geniculate nucleus and thus further from the pial surface than that of every Y-axon. This probably reflects the earlier development of X- than of Y-axons. Furthermore, the parent axon trunks of the X-axons are noticeably thinner than are those of the Y-axons. Every retinogeniculate X- and Y-axon in our sample branches within the optic tract. One of these branches heads dorsally to innervate the lateral geniculate nucleus and one heads medially and rostrally toward the midbrain, although none of these labeled axons were traced to a terminal arbor beyond the lateral geniculate nucleus. For Y-axons, all branches are of comparable diameter, but for X-axons, the branch heading toward the lateral geniculate nucleus is always noticeably thicker than is the branch directed toward the midbrain. 3. Every retinogeniculate X- and Y-axon produces the greatest portion of its terminal arbor in lamina A (if from the contralateral retina) or A1 (if from the ipsilateral retina). These arbors typically extend across most of the lamina along a projection line. Not a single terminal bouton from any axon was found in the inappropriate lamina A or A1 (i.e., in lamina A for ipsilaterally projecting axons or in lamina A1 for contralaterally projecting ones). Occasionally, an X-axon also innervates the medial interlaminar nucleus, and even more rarely does an X-axon innervate the C-laminae. In contrast, nearly all Y-axons from the contralateral retina branch to innervate part of the C-laminae (probably lamina C), and most from either retina also innervate the medial interlaminar nucleus. Although these details imply considerable variation in the overall pattern of retinogeniculate innervation for both X- and Y-axons, we found no physiological properties to correlate with this variation.(ABSTRACT TRUNCATED AT 400 WORDS)