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Monkeypox virus genome sequence from an imported human case in Colombia.
Katherine Laiton-Donato1, Diego A Álvarez-Díaz2, Carlos Franco-Muñoz2
1Grupo Genómica de Microorganismos Emergentes, Dirección de Investigación en Salud Pública, Instituto Nacional de Salud, Bogotá, D.C., Colombia. klaiton@ins.gov.co.
Biomedica : Revista Del Instituto Nacional De Salud
|September 19, 2022
Summary
Genomic sequencing of monkeypox virus (MPXV) from a Colombian case revealed it belongs to Clade IIb. This finding links the Colombian MPXV to the 2022 multi-country outbreak strains.
Area of Science:
- Virology
- Genomics
- Epidemiology
Background:
- Monkeypox virus (MPXV) is a double-stranded DNA virus classified into three clades: Clade I and Clades IIa/IIb.
- Clade IIb strains have been associated with the 2022 multi-country monkeypox outbreaks.
Purpose of the Study:
- To perform complete genome sequencing and phylogenetic analysis of a human monkeypox virus case in Colombia.
- To determine the genetic relationship of the Colombian MPXV strain to other known clades and outbreak strains.
Main Methods:
- Direct genomic sequencing of viral DNA from patient lesion exudate using Nanopore GridION.
- Bioinformatic analysis including read alignment to the MPXV reference genome and phylogenetic inference using maximum likelihood estimation.
Main Results:
- Successful sequencing and assembly of the MPXV genome from the Colombian sample.
- Phylogenetic analysis indicated the Colombian MPXV strain is closely related to Clade IIb.
Conclusions:
- The MPXV circulating in Colombia is linked to the Clade IIb strains responsible for the 2022 global outbreak.
- This study provides crucial genomic data for understanding MPXV transmission dynamics in South America.
