Microvascular effects of oxygen and carbon dioxide measured by vascular occlusion test in healthy volunteers

Changes in near-infrared spectroscopy-derived regional tissue oxygen saturation (StO₂) during a vascular occlusion test (VOT; ischemic provocation of microcirculation by rapid inflation and deflation of a tourniquet) allow estimating peripheral tissue O₂ consumption (desaturation slope; DS), vascular reactivity (recovery slope; RS) and post-ischemic hyperperfusion (AUC-H). The effects of isolated alterations in the inspiratory fraction of O₂ (FiO₂) and changes in expiratory CO₂ remain to be elucidated. Therefore, in this secondary analysis we determined the effects of standardized isolated instances of hypoxia, hyperoxia, hypocapnia and hypercapnia on the VOT-induced StO₂ changes in healthy volunteers (n = 20) to establish reference values for future physiological studies.

StO₂ was measured on the thenar muscle. Multiple VOTs were performed in a standardized manner: i.e. at room air (baseline), during hyperoxia (FiO₂ 1.0), mild hypoxia (FiO₂ ≈ 0.11), and after a second baseline, during hypocapnia (end-tidal CO₂ (etCO₂) 2.5-3.0 vol%) and hypercapnia (etCO₂ 7.0-7.5 vol%) at room air. Differences in DS, RS, and AUC-H were tested using repeated-measures ANOVA.

DS and RS remained constant during all applied conditions. AUC-H after hypoxia was smaller compared to hyperoxia (963 %*sec vs hyperoxia 1702 %*sec, P = 0.005), while there was no difference in AUC-H duration between hypoxia and baseline. The StO₂ peak (after tourniquet deflation) during hypoxia was lower compared to baseline and hyperoxia (92 % vs 94 % and 98 %, P < 0.001).

We conclude that in healthy volunteers at rest, common situations observed during anesthesia and intensive care such as exposure to hypoxia, hyperoxia, hypocapnia, or hypercapnia, did not affect peripheral tissue O₂ consumption and vascular reactivity as assessed by VOT-induced changes in StO₂. These observations may serve as reference values for future physiological studies.

This study represents a secondary analysis of an original study which has been registered at ClinicalTrials.gov nr: NCT02561052.