Plasmid encoding miRNA-200c delivered by CaCO₃-based nanoparticles enhances rat alveolar bone formation

Aim: miRNAs have been shown to improve the restoration of craniofacial bone defects. This work aimed to enhance transfection efficiency and miR-200c-induced bone formation in alveolar bone defects via plasmid DNA encoding miR-200c delivery from CaCO₃ nanoparticles. Materials & methods: The CaCO₃/miR-200c delivery system was evaluated in vitro (microscopy, transfection efficiency, biocompatibility) and miR-200c-induced in vivo alveolar bone formation was assessed via micro-computed tomography and histology. Results: CaCO₃ nanoparticles significantly enhanced the transfection of plasmid DNA encoding miR-200c without inflammatory effects and sustained miR-200c expression. CaCO₃/miR-200c treatment in vivo significantly increased bone formation in rat alveolar bone defects. Conclusion: CaCO₃ nanoparticles enhance miR-200c delivery to accelerate alveolar bone formation, thereby demonstrating the application of CaCO₃/miR-200c to craniofacial bone defects.