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Updated: Aug 28, 2025
A Cell Culture Model for Producing High Titer Hepatitis E Virus Stocks
Published on: June 26, 2020
Hepatitis E virus is effectively inactivated by methylene blue plus light treatment
Ute Gravemann1, Michael Engelmann2, Volker Kinast2,3
1German Red Cross Blood Service NSTOB, Springe, Germany.
Background And Objectives:
Photodynamic treatment with methylene blue (MB) and visible light is a well-established pathogen inactivation system for human plasma. This technique is routinely used in different countries. MB/light treatment was shown to inactivate several transfusion-transmittable viruses, but its efficiency for the inactivation of the quasi-enveloped hepatitis E virus (HEV) has not yet been investigated.
Materials And Methods:
Plasma units were spiked with cell culture-derived HEV and treated with the THERAFLEX MB-Plasma system using various light doses (30, 60, 90, and 120 J/cm2 ). HEV titers in pre- and post-treatment samples were determined by virus titration and a large-volume plating assay to improve the detection limit of the virus assay.
Results:
THERAFLEX MB-Plasma efficiently inactivated HEV in human plasma. Even the lowest light dose of 30 J/cm2 inactivated HEV down to the limit of detection, with a mean log reduction factor of greater than 2.4 for the total process.
Conclusion:
Our study demonstrates that the THERAFLEX MB-Plasma system effectively inactivates HEV in human plasma.
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