Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Overview of Archaea

Overview of Archaea

Archaea, named after the Archaean eon, represent a unique domain of life, distinct from bacteria and eukaryotes, with remarkable traits. Their cellular and molecular features, ecological adaptability, and industrial relevance highlight their importance in understanding life processes and leveraging biotechnology.Cellular and Molecular CharacteristicsA defining feature of archaea is their unique membrane composition. Archaeal membranes contain ether-linked isoprenoid lipids, which confer...

Transformation

Transformation

Microbial communities are dynamic environments where cell lysis releases free DNA into the surroundings. Other cells can take up this extracellular DNA through a process known as transformation.When a cell incorporates this foreign DNA into its genome, resulting in genetic modification, the process is known as transformation. Cells capable of this process are termed competent. Competence can be natural, as observed in certain bacteria and archaea, or artificially induced in the...

Diversity of Archaea III

Diversity of Archaea III

Crenarchaeota, a prominent phylum of Archaea, is remarkable for its ability to thrive in extreme environments characterized by high temperatures and acidity. These microorganisms inhabit sulfuric hot springs, volcanic systems, and submarine hydrothermal vents, where temperatures often exceed 100°C. The unique adaptations of Crenarchaeota not only allow survival under such extreme conditions but also provide insights into the mechanisms of life in primordial Earth-like...

Diversity of Archaea I

Diversity of Archaea I

Archaea, a domain of single-celled microorganisms, are classified into five major phyla based on genetic and biochemical characteristics: Euryarchaeota, Crenarchaeota, Thaumarchaeota, Korarchaeota, and Nanoarchaeota. Among these, the phylum Euryarchaeota is notable for its remarkable diversity in morphology, metabolism, and ecological adaptations.Morphological and Metabolic DiversityMembers of Euryarchaeota exhibit a variety of cellular shapes, including rods and cocci. Their metabolic pathways...

Conservative Site-specific Recombination and Phase Variation

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Has discomfort during neonatal transfers improved over time?

European journal of pediatrics·2026

Same author

Archaeal G-quadruplexes: a novel model for understanding unusual DNA/RNA structures across the tree of life.

Nucleic acids research·2026

Same author

Comparison of volume and non-volume ventilation modes on carbon dioxide following neonatal transport.

Pediatric research·2025

Same author

Gap Analysis Investigating Healthcare Professionals' Skills and Knowledge for Pain Management in Haemophilia-A Qualitative Study.

Haemophilia : the official journal of the World Federation of Hemophilia·2025

Same author

CRISPR-Cas induced self-targeting identifies key players in archaeal microhomology-mediated end joining.

microLife·2025

Same author

Provirus deletion from <i>Haloferax volcanii</i> affects motility, stress resistance, and CRISPR RNA expression.

microLife·2025

Search research articles

Related Experiment Video

Updated: Aug 28, 2025

Genetic Manipulation in Δku80 Strains for Functional Genomic Analysis of Toxoplasma gondii

Genetic Manipulation in Δku80 Strains for Functional Genomic Analysis of Toxoplasma gondii

Published on: July 12, 2013

Progress and Challenges in Archaeal Genetic Manipulation.

Catherine Harrison¹, Thorsten Allers²

¹School of Life Sciences, University of Nottingham, Queen's Medical Centre, Nottingham, UK.

Methods in Molecular Biology (Clifton, N.J.)

|September 20, 2022

Summary

This summary is machine-generated.

This primer reviews model archaeal genetic systems, highlighting challenges and advances in genetic modification. Understanding archaeal genetics offers insights into eukaryotic origins.

Keywords:

Genetics Halophiles Methanogens Sulfolobales Thermococcales

More Related Videos

Site-specific Bacterial Chromosome Engineering: ΦC31 Integrase Mediated Cassette Exchange (IMCE)

Site-specific Bacterial Chromosome Engineering: ΦC31 Integrase Mediated Cassette Exchange (IMCE)

Published on: March 16, 2012

Mapping Bacterial Functional Networks and Pathways in Escherichia Coli using Synthetic Genetic Arrays

Mapping Bacterial Functional Networks and Pathways in Escherichia Coli using Synthetic Genetic Arrays

Published on: November 12, 2012

Related Experiment Videos

Last Updated: Aug 28, 2025

Genetic Manipulation in Δku80 Strains for Functional Genomic Analysis of Toxoplasma gondii

Genetic Manipulation in Δku80 Strains for Functional Genomic Analysis of Toxoplasma gondii

Published on: July 12, 2013

Site-specific Bacterial Chromosome Engineering: ΦC31 Integrase Mediated Cassette Exchange (IMCE)

Site-specific Bacterial Chromosome Engineering: ΦC31 Integrase Mediated Cassette Exchange (IMCE)

Published on: March 16, 2012

Mapping Bacterial Functional Networks and Pathways in Escherichia Coli using Synthetic Genetic Arrays

Mapping Bacterial Functional Networks and Pathways in Escherichia Coli using Synthetic Genetic Arrays

Published on: November 12, 2012

Area of Science:

Microbiology
Molecular Biology
Evolutionary Biology

Background:

Archaea represent diverse habitats and are key to understanding eukaryotic origins.
Model archaeal genetic systems are crucial for advancing biological research.
Genetic tractability in archaea remains a significant research area.

Purpose of the Study:

To review available model archaeal genetic systems.
To discuss limitations and barriers in archaeal genetic modification.
To highlight techniques and breakthroughs in archaeal genetic tractability.

Main Methods:

Literature review of existing archaeal genetic systems.
Analysis of challenges and successes in genetic manipulation of archaea.
Identification of future research directions for archaeal genetics.

Main Results:

Several archaeal species serve as model systems for genetic studies.
Significant progress has been made in overcoming genetic modification barriers.
Specific techniques have enhanced the tractability of certain archaeal species.

Conclusions:

Model archaeal genetic systems are essential for studying archaeal biology and evolution.
Continued development in genetic techniques will improve archaeal tractability.
Further research into archaeal genetics will illuminate eukaryotic origins.