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Updated: Aug 28, 2025

Annotation of Plant Gene Function via Combined Genomics, Metabolomics and Informatics
Published on: June 17, 2012
Protocol for gene annotation, prediction, and validation of genomic gene expansion
Quanwei Zhang1, Zhengdong D Zhang1
1Department of Genetics, Albert Einstein College of Medicine, Bronx, NY, USA.
Abstract:
Although gene expansion plays an important role in evolution, its identification remains a challenge due to potential errors in genome assembly and annotation. Here, we describe a detailed step-by-step protocol for gene annotation, prediction of genomic gene expansion, and its computational and experimental validation. Finally, we also detail steps to discover functionality of each copy of replicated genes. For complete details on the use and execution of this protocol, please refer to Zhang et al. (2021).
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