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DNA hydrogels combined with microfluidic chips for melamine detection.

Zhiguang Wang1, Ruipeng Chen2, Yue Hou1

  • 1College of Chemistry and Materials Science, Shanghai Normal University, Shanghai, 200234, China; Tianjin Key Laboratory of Risk Assessment and Control Technology for Environment and Food Safety, Institute of Environmental and Operational Medicine, Tianjin, 300050, China.

Analytica Chimica Acta
|September 20, 2022
PubMed
Summary

A new point-of-care testing (POCT) method uses DNA hydrogels and microfluidic chips for sensitive melamine detection. This portable technology enables rapid field testing for food safety and clinical applications.

Keywords:
DNA hydrogelMelamine detectionMicrofluidic chip

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Area of Science:

  • Analytical Chemistry
  • Materials Science
  • Biotechnology

Background:

  • Illegal melamine (MEL) adulteration in food products like pet food and infant formula poses significant public health risks.
  • Existing detection methods often require complex laboratory equipment, limiting their use in rapid field screening.
  • Development of portable, sensitive, and user-friendly detection systems is crucial for effective monitoring and prevention.

Purpose of the Study:

  • To develop a novel point-of-care testing (POCT) method for the sensitive and portable detection of melamine.
  • To integrate stimuli-responsive deoxyribonucleic acid (DNA) hydrogels with microfluidic chips for enhanced melamine analysis.
  • To provide a user-friendly field testing solution for melamine detection, applicable to food security and clinical diagnostics.

Main Methods:

  • Fabrication of DNA hydrogels cross-linked with melamine aptamers (MA) and coated with gold nanoparticles (AuNPs).
  • Utilized the competitive binding of melamine to the aptamer, leading to DNA hydrogel disintegration and AuNP release for colorimetric detection.
  • Integrated the DNA hydrogel system with microfluidic chips for quantitative analysis via image-based gray value measurement.

Main Results:

  • The DNA hydrogel method achieved a detection range of 0.1-100 μM for melamine with a limit of detection (LOD) of 42 nM.
  • The microfluidic chip-based method demonstrated a detection range of 0.2-50 μM for melamine with an LOD of 37 nM.
  • The developed system is portable, sensitive, and does not require trained operators or large laboratory instruments.

Conclusions:

  • The combined DNA hydrogel and microfluidic chip approach offers a promising POCT solution for rapid and accurate melamine detection.
  • This method facilitates visual and quantitative analysis, making it suitable for on-site food safety, clinical, and environmental monitoring.
  • The user-friendly nature and portability of the system highlight its potential for widespread application in public health surveillance.