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Updated: Aug 28, 2025

Development of an Electrochemical DNA Biosensor to Detect a Foodborne Pathogen
Published on: June 3, 2018
Multiplexed and DNA amplification-free detection of foodborne pathogens in egg samples: Combining electrical
Xiaobo Hu1, Xinrui Cheng1, Zhilong Wang1
1Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Ministry of Education, Wuhan, 430070, Hubei, China.
Abstract:
A multiplex and DNA amplification-free sensor based on electrical resistance microsphere counter (ERMC) and DNA hybridization reaction has been developed for simultaneous detection of Listeria monocytogenes, Salmonella Typhimurium, and Staphylococcus aureus in egg samples. In this strategy, magnetic nanoparticles (MNPs) and polystyrene microspheres (PMs) were labeled with DNA probes, the PMs with different sizes (3 μm, 4 μm and 6 μm) were used as multiplex signal reporters due to the high resolution of ERMC. After magnetic separation, the number of unbound PM-probe was related to the concentration of pathogens, and their sizes were attributed to types of pathogens. The limit of detection of this sensor was 20 CFU/mL for Listeria monocytogenes, 89 CFU/mL for Salmonella, and 94 CFU/mL for Staphylococcus aureus, respectively. The DNA amplification-free, simplicity and low-cost of this assay make it a promising tool for the rapid screening of multiple pathogens in food samples.

