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Modulation of radiosensitivity of DU145 prostate carcinoma cells by simvastatin
Verena Korte1, Guenther Gademann1, Ahmed Gawish2,3
1Laboratory for Radiation Biology, Clinic for Radiotherapy, Medical Faculty, Otto-von-Guericke-University, Magdeburg, Germany.
Purpose:
To investigate antiproliferative effects of simvastatin in combination with ionizing radiation on DU145 prostate cancer cells and its influence on cellular HMG-CoA-reductase levels.
Methods:
Proliferative responses of DU145 cells were estimated by means of a clonogenic assay or the crystal violet procedure. HMG-CoA-reductase levels were measured by western blot analysis.
Results:
The antiproliferative effects of simvastatin and radiation are dependent on simvastatin dose, radiation dose and treatment time. In vitro treatment of DU145 cells with simvastatin induced HMG-CoA-reductase levels.
Conclusion:
Ionizing radiation more profoundly reduces proliferation as compared to simvastatin exposure, while the combined application of both modalities is synergistic. The inhibition of CoA-reductase may contribute to these effects.
