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Related Experiment Video

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Precision Cut Lung Slices as an Efficient Tool for Ex vivo Pulmonary Vessel Structure and Contractility Studies
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Functional changes in long-term incubated rat precision-cut lung slices.

Sarah Marie Nußbaum1, Julia Krabbe2, Svenja Böll3

  • 1Institute of Pharmacology and Toxicology, Medical Faculty, RWTH Aachen University, Wendlingweg 2, 52074, Aachen, Germany.

Respiratory Research
|September 20, 2022
PubMed
Summary

Long-term cultivation of rat precision-cut lung slices (PCLS) over 29 days was successful, maintaining viability and reactivity for at least 13 days. This model shows promise for studying chronic respiratory diseases and toxicity.

Keywords:
BronchoconstrictionFBSFetal bovine serumGene expressionHistopathologyLong-term incubationPCLSPrecision-cut lung slicesRatViability

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Area of Science:

  • * Respiratory research utilizing ex vivo models.
  • * Development of advanced cell culture techniques for disease modeling.

Background:

  • * Respiratory diseases pose a significant global health challenge.
  • * Precision-cut lung slices (PCLS) offer a valuable ex vivo alternative to animal models for studying airway diseases.
  • * Previous research focused on short-term PCLS cultivation (≤72 hours), with recent advancements extending to 4 weeks for human PCLS.

Purpose of the Study:

  • * To establish and validate a long-term cultivation model of rat PCLS over 29 days.
  • * To assess the viability, histopathology, reactivity, and gene expression of rat PCLS during extended culture periods.
  • * To evaluate the feasibility of using long-term PCLS for chronic disease and toxicity research.

Main Methods:

  • * Rat PCLS were cultured for 29 days.
  • * Analysis included viability, histopathology, and reactivity assessments at various time points.
  • * Gene expression (mRNA) patterns were analyzed throughout the cultivation period.

Main Results:

  • * Successful 29-day cultivation of rat PCLS with sustained viability.
  • * Bronchoconstriction capacity was maintained for 13-25 days, though relaxation and sensitivity were altered.
  • * Gene expression patterns changed during long-term culture; fetal bovine serum (FBS) addition negatively impacted viability and introduced cellular changes.

Conclusions:

  • * A viable and reactive rat PCLS model for up to 29 days was established.
  • * The model remains reactive for at least 13 days, suitable for certain research applications.
  • * Further research is needed to optimize long-term PCLS for chronic disease and toxicity studies.