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Microtubule structure at 18 A resolution.

L Beese, G Stubbs, C Cohen

    Journal of Molecular Biology
    |March 20, 1987
    PubMed
    Summary
    This summary is machine-generated.

    A new model reveals microtubule structure at 18 angstroms resolution, detailing protofilament grooves and tubulin domain organization. This provides insights into the flexibility and polymorphism of tubulin assemblies.

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    Area of Science:

    • Biophysics
    • Structural Biology
    • Cell Biology

    Background:

    • Microtubules are essential cytoskeletal components involved in cell structure and division.
    • Understanding microtubule structure is key to deciphering their dynamic functions.

    Purpose of the Study:

    • To determine the three-dimensional structure of microtubules at high resolution.
    • To investigate the structural basis for tubulin assembly polymorphism.

    Main Methods:

    • X-ray fiber diffraction of hydrated, reassembled calf brain microtubules.
    • Iterative solvent flattening refinement with electron microscopy-derived phases.

    Main Results:

    • A structural model of microtubules at 18 angstroms resolution was generated.

    Related Experiment Videos

  • The model shows protofilaments forming a hollow cylinder with defined surface grooves.
  • Electron density fluctuations suggest a domain structure within tubulin subunits.

    • Conclusions:

    • The proposed tubulin domain structure explains microtubule flexibility.
    • Variations in domain arrangement can account for the observed polymorphism in tubulin assemblies.