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Related Concept Videos

Matrix-Assisted Laser Desorption Ionization (MALDI)01:08

Matrix-Assisted Laser Desorption Ionization (MALDI)

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Matrix-assisted laser desorption ionization (MALDI) is a powerful analytical technique used in mass spectrometry. It enables the identification and characterization of various biomolecules, including proteins, peptides, nucleic acids, and carbohydrates. MALDI spectrometry is widely employed in biological and medical research, as well as in fields like pharmacology and biochemistry.
The analyte of interest, a biomolecule or a mixture of biomolecules, is mixed with a suitable matrix material. The...
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MALDI-TOF Mass Spectrometry01:19

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Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.
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Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

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Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
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Electrospray Ionization (ESI) Mass Spectrometry01:12

Electrospray Ionization (ESI) Mass Spectrometry

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Higher molecular weight biomolecules are nonvolatile compounds that may decompose before ionizing or vaporizing during mass analysis with conventional electron impact ionization methods. Accordingly, electrospray ionization (ESI) is the favored method for vaporizing and ionizing biomolecules as it circumvents rapid fragmentation and enables the recording of mass signals for the entire biomolecule.
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
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Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview01:19

Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview

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In inductively coupled plasma–mass spectrometry (ICP–MS), an inductively coupled plasma (ICP) torch is used as an atomizer and ionizer. Solid samples are dissolved and volatilized before being introduced into the high-temperature argon plasma, while solution samples are nebulized and passed through the high-temperature argon plasma. Plasma dissociates the analytes and ionizes their component atoms to form a mixture of positive ions and molecular species. The positive ions are then...
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Related Experiment Video

Updated: Aug 28, 2025

Matrix-assisted Laser Desorption/Ionization Time of Flight MALDI-TOF Mass Spectrometric Analysis of Intact Proteins Larger than 100 kDa
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High-Throughput Intact Protein Analysis for Drug Discovery Using Infrared Matrix-Assisted Laser Desorption

Fan Pu1, Scott A Ugrin1, Andrew J Radosevich1

  • 1AbbVie Inc, 1 North Waukegan Rd., North Chicago, Illinois 60064, United States.

Analytical Chemistry
|September 21, 2022
PubMed
Summary
This summary is machine-generated.

Infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI-MS) offers a high-throughput solution for intact protein analysis in biopharmaceuticals. This ambient ionization MS technology achieves significantly faster sample rates than traditional LC/MS, enabling rapid drug discovery workflows.

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Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization IR-MALDESI
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Low Molecular Weight Protein Enrichment on Mesoporous Silica Thin Films for Biomarker Discovery
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Last Updated: Aug 28, 2025

Matrix-assisted Laser Desorption/Ionization Time of Flight MALDI-TOF Mass Spectrometric Analysis of Intact Proteins Larger than 100 kDa
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Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization IR-MALDESI
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Low Molecular Weight Protein Enrichment on Mesoporous Silica Thin Films for Biomarker Discovery
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Low Molecular Weight Protein Enrichment on Mesoporous Silica Thin Films for Biomarker Discovery

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Area of Science:

  • Analytical Chemistry
  • Biotechnology
  • Mass Spectrometry

Background:

  • Mass spectrometry (MS) is crucial for protein characterization in biopharmaceuticals.
  • Liquid chromatography coupled with electrospray ionization (LC/ESI-MS) is the standard for intact protein analysis but is limited by speed.
  • High-throughput analysis (>1000 samples/day) is essential for modern drug discovery.

Purpose of the Study:

  • To evaluate infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI-MS) for high-throughput intact protein analysis.
  • To assess the system's performance with various protein concentrations, molecular weights, and buffer conditions.
  • To demonstrate the coupling of sample cleanup methods for complex matrices.

Main Methods:

  • Intact protein analysis using an ambient ionization IR-MALDESI-MS system.
  • Testing a wide range of protein concentrations (low to high) and molecular weights (10-150 kDa).
  • Coupling multiplexed plate-based sample cleanup methods with IR-MALDESI-MS.

Main Results:

  • IR-MALDESI-MS detected proteins from 10-150 kDa with enhanced tolerance to salts and buffers compared to ESI.
  • Achieved sample rates up to 22 Hz for high concentrations and 1.5 Hz for low concentrations in assay buffers.
  • Demonstrated a ~22x speed increase over current high-throughput ESI-MS methods and enabled real-time monitoring of biological processes.

Conclusions:

  • IR-MALDESI-MS is a viable high-throughput platform for intact protein analysis in drug discovery.
  • The system offers improved speed and robustness, overcoming limitations of traditional LC/ESI-MS.
  • Coupling with cleanup methods expands its applicability to complex biological samples.