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Fluorescence detection methods for microfluidic droplet platforms
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A protocol to study microdroplet photoreaction at an individual droplet level using in situ micro-Raman spectroscopy

Kejian Li1, Longqian Wang2, Juan Liu2

  • 1Shanghai Key Laboratory of Atmospheric Particle Pollution and Prevention, Department of Environmental Science and Engineering, Fudan University, Shanghai 200433, People's Republic of China; Shanghai Institute of Pollution Control and Ecological Security, Shanghai 200092, People's Republic of China.

STAR Protocols
|September 21, 2022
PubMed

Keywords:
ChemistryEnergyEnvironmental sciencesMaterial sciencesPhysics

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Raman Spectroscopy Instrumentation: Overview01:26

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A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
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