Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

<i>In Situ</i> Mineralization of Gold Nanoparticles from Sodium Aurothiomalate or Tetrachloroauric Acid in Human Cells.

ACS nanoscience Au·2026
Same author

A computational analysis of the glycoprotein LRP1 structure and the role of glycans as quaternary glue.

Bioinformatics (Oxford, England)·2026
Same author

STORM as a tool to track cargo release from polymeric nanocarriers at the single-particle level.

Nanoscale horizons·2026
Same author

Light and Enzymatic Cooperative Response in Supramolecular Fibers: A Synergistic Strategy for Potential Drug Delivery Applications.

Biomacromolecules·2026
Same author

Phenotyping by Molecular Mobility: Low-Affinity Reversible Probes Enable Characterizing and Classifying Cells Populations by Single-Molecule Imaging.

Journal of the American Chemical Society·2026
Same author

Tunable PEGylated albumin nanocarriers enhance 5-FU cytotoxic selectivity and modulate oxidative and immune stress in colorectal cancer model.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie·2026

Related Experiment Video

Updated: Aug 28, 2025

Quantification of Endosome and Lysosome Motilities in Cultured Neurons Using Fluorescent Probes
08:15

Quantification of Endosome and Lysosome Motilities in Cultured Neurons Using Fluorescent Probes

Published on: May 22, 2017

8.2K

Nanoscopy for endosomal escape quantification.

Teodora Andrian1, Roger Riera2, Silvia Pujals1,3

  • 1Nanoscopy for Nanomedicine, Institute for Bioengineering of Catalonia Barcelona Spain l.albertazzi@tue.nl.

Nanoscale Advances
|September 22, 2022
PubMed
Summary

Nanoparticle drug delivery requires overcoming endosomal entrapment. Nanoscopy offers advanced imaging to quantify nanoparticle endosomal escape, crucial for developing smarter nanomedicines.

More Related Videos

Using Nanoplasmon-Enhanced Scattering and Low-Magnification Microscope Imaging to Quantify Tumor-Derived Exosomes
09:30

Using Nanoplasmon-Enhanced Scattering and Low-Magnification Microscope Imaging to Quantify Tumor-Derived Exosomes

Published on: May 24, 2019

7.5K
The Microscopy-Based Assay to Study and Analyze the Recycling Endosomes using SNARE Trafficking
08:51

The Microscopy-Based Assay to Study and Analyze the Recycling Endosomes using SNARE Trafficking

Published on: February 12, 2022

3.7K

Related Experiment Videos

Last Updated: Aug 28, 2025

Quantification of Endosome and Lysosome Motilities in Cultured Neurons Using Fluorescent Probes
08:15

Quantification of Endosome and Lysosome Motilities in Cultured Neurons Using Fluorescent Probes

Published on: May 22, 2017

8.2K
Using Nanoplasmon-Enhanced Scattering and Low-Magnification Microscope Imaging to Quantify Tumor-Derived Exosomes
09:30

Using Nanoplasmon-Enhanced Scattering and Low-Magnification Microscope Imaging to Quantify Tumor-Derived Exosomes

Published on: May 24, 2019

7.5K
The Microscopy-Based Assay to Study and Analyze the Recycling Endosomes using SNARE Trafficking
08:51

The Microscopy-Based Assay to Study and Analyze the Recycling Endosomes using SNARE Trafficking

Published on: February 12, 2022

3.7K

Area of Science:

  • Nanomedicine
  • Biotechnology
  • Advanced Microscopy

Background:

  • Nanoparticle cytosolic delivery is hindered by endosomal entrapment and degradation.
  • Current methods for detecting nanoparticle endosomal escape lack nanoscale quantification.
  • Improved detection is vital for the rational design of effective nanomedicines.

Purpose of the Study:

  • To review the application of nanoscopy techniques for studying nanoparticle endosomal escape.
  • To highlight the potential of nanoscopy in advancing nanomedicine research.
  • To discuss the features of nanoscopy that enable impactful studies in drug delivery.

Main Methods:

  • Review of recent literature on nanoscopy applications in nanomedicine.
  • Focus on techniques providing high resolution and specificity for nanoscale imaging.
  • Analysis of how nanoscopy addresses limitations in quantifying endosomal escape.

Main Results:

  • Nanoscopy provides unprecedented resolution and specificity for visualizing nanoparticle-cell interactions.
  • Emerging nanoscopy techniques offer quantitative insights into endosomal escape mechanisms.
  • These advanced imaging methods are crucial for understanding nanoparticle trafficking and intracellular delivery.

Conclusions:

  • Nanoscopy is a powerful tool for elucidating nanoparticle endosomal escape.
  • Implementing advanced nanoscopy will significantly impact the development of smart nanoparticles.
  • Further integration of nanoscopy is essential for optimizing nanomedicine delivery strategies.