Rutile nano-bio-interactions mediate dissimilar intracellular destiny in human skin cells
P L Sanches1,2,3, W Souza1,3,4, S Gemini-Piperni3,5
1Directory of Life Sciences Applied Metrology, National Institute of Metrology Quality and Technology Av. Nossa Senhora das Graças 50 Xérem Rio de Janeiro Brazil.
Nanoscale Advances
|September 22, 2022
Summary
Titanium dioxide nanoparticles (TiO2 NPs) interact with skin cells, forming bio-complexes that influence internalization and autophagy. Intracellular trafficking differs between fibroblasts and keratinocytes, with NPs found in multivesicular bodies in keratinocytes.
Area of Science:
- Nanomedicine
- Dermatology
- Toxicology
Background:
- Nanoparticles (NPs) are increasingly used in healthcare due to unique properties.
- Titanium dioxide NPs (TiO2 NPs) are common in sunscreens but their skin cell interactions are poorly understood.
- Investigating nanoscale biological interactions is crucial for understanding NP safety.
Purpose of the Study:
- To determine if initial nano-bio-complex formation predicts TiO2 NP internalization and trafficking in human skin cells.
- To explore the impact of nano-bio-interactions on cell viability and proliferation.
- To investigate the role of multivesicular bodies (MVBs) in NP intracellular transport.
Main Methods:
- Primary human fibroblasts and keratinocytes were exposed to TiO2 NPs.
- Nano-bio-complex formation was analyzed.
- Cell viability, proliferation, and intracellular trafficking were assessed.
- Autophagy and NP localization within cellular compartments were examined.
Main Results:
- TiO2 NPs did not significantly affect cell viability but may compromise proliferation.
- Bio-complex formation, dependent on media chemistry and NP properties, facilitated NP uptake and induced autophagy.
- Intracellular trafficking of NPs differed between fibroblasts and keratinocytes.
- TiO2 NPs were observed within multivesicular bodies (MVBs) in keratinocytes.
Conclusions:
- Initial nano-bio-complex formation is a key factor in TiO2 NP internalization and cellular response.
- NPs can alter exosome biogenesis via MVBs, potentially leading to nanotoxicity.
- Understanding NP-cell interactions is vital for safe application in dermatology and nanomedicine.
