Clearance of single-wall carbon nanotubes from the mouse lung: a quantitative evaluation

Based on the characteristics of carbon nanotubes (CNTs) that absorb light in the near-infrared region, we have developed a method to quantify the biodistribution of CNTs in mouse tissues such as the liver, lungs and spleen. By using this method, the kinetic biodistribution of single-walled CNTs (SWNTs) after intravenous injection into mice for 60 days has been successfully investigated. The results show that the biodistribution of CNTs was diameter-dependent by comparing two different diameters of SWNTs. The SWNTs with larger diameters (1-5 nm) accumulated more in the liver or spleen but less in the lungs than those with smaller diameters (0.7-0.9 nm). The quantities of both SWNTs in the liver and lungs decreased with time and showed no significant change in the spleen, which is also confirmed by histological analysis. In particular, the results have demonstrated that both SWNTs are cleared from the lungs almost completely within 60 days, suggesting that the pulmonary toxicity of SWNTs would be low when low amounts of CNTs (<70 μg g^-1 of tissue) enter inside the lungs. In addition, no obvious inflammatory responses are found from the measurement of the cytokines TGF-β1, IL-6, INF-γ, and TNF-α in the plasma and organs after the injection of both SWNTs into mice.