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Related Experiment Video

Updated: Aug 28, 2025

Synthesis, Cellular Delivery and In vivo Application of Dendrimer-based pH Sensors
16:19

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Published on: September 10, 2013

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Intracellular ratiometric temperature sensing using fluorescent carbon dots.

Jun-Ray Macairan1, Dilan B Jaunky2, Alisa Piekny2

  • 1Department of Chemistry and Biochemistry, Center for NanoScience Research, Concordia University Montreal QC Canada H4B 1R6 rafik.naccache@concordia.ca.

Nanoscale Advances
|September 22, 2022
PubMed
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Researchers developed a new ratiometric temperature sensing method using dual-emitting carbon dots. This approach offers precise, non-invasive temperature measurement for biological applications, overcoming limitations of existing fluorescence-based thermometers.

Area of Science:

  • Nanotechnology
  • Biomedical Engineering
  • Materials Science

Background:

  • Non-invasive temperature sensing is crucial for biological studies and medical diagnostics.
  • Nanoscale thermometers offer subcellular resolution but often face limitations with fluorescence intensity or complex systems.
  • Existing methods require peak deconvolution or hybrid systems, limiting their practical application.

Purpose of the Study:

  • To develop a highly sensitive, non-invasive ratiometric temperature sensing approach using carbon dots.
  • To overcome the limitations of intensity-based fluorescence thermometers.
  • To enable precise temperature monitoring in biological systems.

Main Methods:

  • Synthesized dual-emitting carbon dots (emitting blue and red fluorescence) via microwave synthesis.

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  • Utilized a ratiometric fluorescence-based approach to measure temperature changes.
  • Tested the sensing capabilities in vitro across a temperature range of 5-60 °C and in HeLa cancer cells (32-42 °C).

    • Main Results:

    • Demonstrated a linear temperature response from 5-60 °C with high thermal resolution (0.048 K⁻¹) and sensitivity (1.97% °C⁻¹).
    • Observed temperature-dependent fluorescence changes in HeLa cancer cells, reflecting intracellular temperature variations.
    • Showcased the ratiometric method's independence from probe concentration, unlike intensity-based methods.

    Conclusions:

    • Dual-emitting carbon dots provide a superior ratiometric approach for fluorescence nanothermometry.
    • This method is robust against variations in probe concentration, enhancing reliability.
    • The developed carbon dots show significant potential for in vitro and in vivo temperature sensing applications.