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Bacteria-mediated tumor immunotherapy via photothermally-programmed PD1 expression
Wenxuan Xu1, Debao Ren1, Zimeng Yu1
1State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei Key Laboratory of Industrial Biotechnology, School of Life Sciences, Hubei University Wuhan 430062 P. R. China malixing@hubu.edu.cn huyunhong@hubu.edu.cn.
Nanoscale Advances
|September 22, 2022
Summary
Thermally-induced bacteria (TIB) were engineered to express programmed cell death protein 1 (PD1) in tumors. This approach combines photothermal therapy and immune activation to inhibit tumor growth and metastasis.
Area of Science:
- Oncology
- Immunotherapy
- Biotechnology
Background:
- Solid tumors create a unique microenvironment favoring anaerobic bacteria.
- Intratumoral bacterial infections can disrupt tumor vasculature, leading to increased near-infrared absorption.
Purpose of the Study:
- To develop bacteria-based therapy for enhanced tumor treatment.
- To investigate the combined effects of photothermal therapy and immune activation.
Main Methods:
- Construction of thermally-induced bacteria (TIB) to express programmed cell death protein 1 (PD1).
- Application of laser irradiation to induce TIB-mediated PD1 expression and photothermal effects.
- Evaluation of PD1-PD-L1 binding, immune suppression relief, and anti-tumor efficacy.
Main Results:
- Laser irradiation at tumor sites induced PD1 expression and caused tumor cell damage.
- Upregulated PD1 effectively bound to PD-L1, reducing immune suppression.
- The TIB@PD1 approach significantly inhibited tumor cell proliferation and metastasis.
Conclusions:
- Bacteria-based photothermal immunotherapy offers a novel strategy for cancer treatment.
- The TIB@PD1 system demonstrates good specificity and selectivity for tumor therapy.
