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In Vitro Interactions between Okadaic Acid and Rat Gut Microbiome
Yang Liu1, Siyuan Xu1, Qiudie Cai1
1Key Laboratory of Aquatic Eutrophication and Control of Harmful Algal Blooms of Guangdong Higher Education Institute, College of Life Science and Technology, Jinan University, Guangzhou 510632, China.
Marine Drugs
|September 22, 2022
Summary
Okadaic acid (OA) alters gut bacteria composition and metabolism in vitro. This marine biotoxin influences bacterial populations and produces metabolites, suggesting a direct interaction with the gut microbiome.
Area of Science:
- Marine Biology
- Microbiology
- Toxicology
Background:
- Okadaic acid (OA) is a marine biotoxin causing diarrhetic shellfish poisoning (DSP).
- The complex oral toxicity and precise mechanism of OA remain unclear.
- Gut microbiota interactions may explain OA's complex toxicity.
Purpose of the Study:
- To investigate the in vitro interaction between OA and gut bacteria.
- To explore OA's effect on bacterial composition and metabolism.
- To elucidate OA's role in gut microbiome dynamics.
Main Methods:
- In vitro fermentation of gut bacteria with varying OA concentrations (0-1000 nM).
- 16S rDNA sequencing to analyze bacterial composition changes.
- LC-MS/MS to detect differential metabolites.
Main Results:
- OA modulated bacterial composition, inhibiting some genera while promoting others.
- Eight potential OA metabolites, including dinophysistoxin-2 (DTX-2), were identified.
- The abundance of *Faecalitalea* correlated with OA metabolites, suggesting its involvement in OA metabolism.
Conclusions:
- OA directly interacts with gut bacteria in vitro.
- This interaction influences bacterial composition and metabolite production.
- Findings provide evidence for OA's metabolic pathways and complex toxicity mechanisms.
