Related Experiment Video
Updated: Aug 28, 2025
06:38
Screening and Isolation of C-Glycoside-Cleaving Intestinal Bacteria
Published on: February 28, 2025
433
Actinoflavosides B-D, Flavonoid Type Glycosides from Tidal Mudflat-Derived Actinomyces
Hyeongju Jeong1, Se Jin Jo1, Munhyung Bae2
1Research Institute of Pharmaceutical Sciences, College of Pharmacy, Chonnam National University, Gwangju 61186, Korea.
Marine Drugs
|September 22, 2022
Summary
Three new compounds, actinoflavosides B-D, were found in actinomycete bacteria. These compounds show antibacterial properties and one enhances immune responses.
Area of Science:
- Microbiology
- Natural Products Chemistry
- Immunology
Background:
- Actinomycetes are a rich source of novel bioactive secondary metabolites.
- Marine environments, such as tidal mudflats, harbor diverse microbial communities with unique chemical biosynthetic potential.
Purpose of the Study:
- To isolate and characterize new secondary metabolites from actinomycete strains.
- To evaluate the biological activities of the isolated compounds, including antibacterial and immunomodulatory effects.
Main Methods:
- Isolation and cultivation of actinomycete strains (JML48 and JMS33) from marine sediment.
- Structure elucidation of secondary metabolites using Mass Spectrometry (MS), Ultraviolet (UV) spectroscopy, and Nuclear Magnetic Resonance (NMR) analyses.
- Stereochemical determination of the aminosugar moiety using J-based configuration analysis and modified Mosher's method.
Main Results:
- Three new secondary metabolites, named actinoflavosides B-D (compounds 1-3), were identified.
- Actinoflavosides B-D exhibited antibacterial activity against Pseudomonas aeruginosa.
- Actinoflavoside D demonstrated a significant increase in Interleukin-2 (IL-2) production in mouse splenocytes.
Conclusions:
- The study successfully isolated and characterized novel actinoflavosides from marine actinomycetes.
- The identified compounds possess promising antibacterial and immunomodulatory properties, warranting further investigation.
- Marine actinomycetes represent a valuable resource for the discovery of new therapeutic agents.
Keywords:
actinoflavosideantibacterialflavonoidimmune modulatorsecondary metabolitestructural determinationtidal mudflatMore Related Videos
13:35Structural Biology and Analytical Chemistry Approaches for Characterizing C-Glycoside Metabolic Enzymes in Human Gut Microbiota
Published on: May 23, 2025
567
08:20Systematic Approach to Identify Novel Antimicrobial and Antibiofilm Molecules from Plants' Extracts and Fractions to Prevent Dental Caries
Published on: March 31, 2021
6.4K
Related Concept Videos
01:30Bacterial Phylum Actinobacteria
95
Coryneform bacteria are gram-positive, aerobic, nonmotile rods that exhibit irregular, club-shaped, or V-shaped arrangements. Their V-shape results from snapping division, where the inner cell wall layer forms the cross-wall, while the outer layer remains intact until it ruptures on one side, causing the daughter cells to bend away.The primary genera are Corynebacterium and Arthrobacter. Corynebacterium includes diverse species, ranging from saprophytes to pathogens like Corynebacterium...
95
01:26Bacterial Phylum Bacteroidota
100
The phylum Bacteroidota includes over 700 species classified into four primary orders: Bacteroidales, Cytophagales, Flavobacteriales, and Sphingobacteriales. These gram-negative, non-sporulating rods exhibit saccharolytic capabilities and can be aerobic or fermentative, encompassing obligate aerobes, facultative aerobes, and obligate anaerobes. Many species display gliding motility, though some are nonmotile or use flagella. The genus Bacteroides is well-studied due to its significant role in...
100
01:29Bacterial Flora of the Large Intestine
594
The gut microbiome is formed by a vast and diverse community of bacteria that colonizes our large intestine. These bacteria start residing in the gut from birth and continue diversifying throughout life, influenced by factors such as diet, lifestyle, and stress. The gut bacterial community also includes bacteria from food and those that enter the colon through the anus.
The normal gut flora of the colon plays a critical role in generating essential vitamins such as vitamins K, B5, and B7.
The normal gut flora of the colon plays a critical role in generating essential vitamins such as vitamins K, B5, and B7.
594
01:26Introduction to Actin
5.3K
Actin is a highly conserved cytoskeletal protein found abundantly in eukaryotic cells. It constitutes 10% weight of the total cellular protein in muscle cells, while in non-muscle cells, it is lower and makes up around 1–5 percent of the total cell protein. Actin found in the unicellular amoebae and complex multicellular animals is around 80% similar, demonstrating their conservation over a billion years of evolution. Actin coding genes are conserved within species and across...
5.3K
01:18Actin Treadmilling
8.2K
Actin filaments undergo polymerization and depolymerization from either end. The polymerization and depolymerization rates depend on the cytosolic concentration of free G-actins. The polymerization rate is generally higher at the plus or barbed end, while the depolymerization rate is higher at the minus or pointed end. At a steady state, critical concentration describes the concentration of free G-actin monomers at which the polymerization rate at the plus end is equal to that of the...
8.2K