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Plasma Concentrations of Vinculin versus Talin-1 in Coronary Artery Disease
Masayuki Aoyama1,2, Yoshimi Kishimoto3, Emi Saita4
1Department of Cardiology, NHO Tokyo Medical Center, Tokyo 152-8902, Japan.
Insights
Plasma vinculin concentrations are not elevated in patients with coronary artery disease (CAD). Unlike talin-1, vinculin levels did not correlate with CAD presence or severity, suggesting it is not a reliable biomarker for this condition.
Area of Science:
- Cardiovascular Biology
- Molecular Medicine
- Biomarker Discovery
Background:
- Cytoskeletal proteins vinculin and talin-1 are implicated in focal adhesions and have been observed at lower levels in atherosclerotic lesions.
- Previous research indicated elevated plasma talin-1 concentrations in patients with coronary artery disease (CAD).
- The role and concentration of plasma vinculin in CAD patients remained unclarified.
Purpose of the Study:
- To investigate plasma vinculin concentrations in patients undergoing coronary angiography.
- To compare plasma vinculin and talin-1 levels between patients with and without CAD.
- To assess the association between plasma vinculin levels and the severity of CAD.
Main Methods:
- Plasma samples were collected from 327 patients undergoing coronary angiography.
- Coronary artery disease (CAD) was diagnosed and categorized by the number of affected vessels (1-, 2-, or 3-vessel disease).
- Plasma concentrations of vinculin and talin-1 were measured and statistically analyzed.
Main Results:
- Plasma vinculin concentrations did not significantly differ between patients with and without CAD (p=0.325) or across different CAD severities (p=0.202).
- In contrast, plasma talin-1 concentrations were significantly higher in CAD patients (p=0.006) and showed a stepwise increase with the number of stenotic vessels (p=0.043).
- No correlation was found between plasma vinculin and talin-1 levels, and multivariate analysis indicated vinculin is not a predictor of CAD.
Conclusions:
- Plasma vinculin concentrations are not elevated in patients with coronary artery disease (CAD).
- Plasma vinculin levels are not associated with the presence or severity of CAD.
- Unlike talin-1, vinculin does not appear to be a useful biomarker for CAD.
Abstract:
Vinculin and talin-1, which are cytoskeletal proteins affecting focal adhesions, were reported to be down-expressed in atherosclerotic lesions. Recently, we reported high concentrations of plasma talin-1 in patients with coronary artery disease (CAD). However, blood vinculin concentrations in CAD patients have not been clarified. Plasma vinculin concentrations as well as talin-1 were studied in 327 patients in whom coronary angiography was performed. CAD was proven in 177 patients (1-vessel, n = 79; 2-vessel, n = 57; 3-vessel disease, n = 41). However, vinculin concentrations were not markedly different between the CAD(-) and CAD groups (median 122.5 vs. 119.6 pg/mL, p = 0.325) or among patients with CAD(-), 1-, 2-, and 3-vessel diseases (122.5, 112.8, 107.9, and 137.2 pg/mL, p = 0.202). In contrast, talin-1 concentrations were higher in CAD than the CAD(-) group (0.29 vs. 0.23 ng/mL, p = 0.006) and increased stepwise in the number of stenotic vessels: 0.23 in CAD(-), 0.28 in 1-vessel, 0.29 in 2-vessel, and 0.33 ng/mL in 3-vessel disease (p = 0.043). No correlation was observed between vinculin and talin-1 concentrations. In multivariate analysis, vinculin concentrations were not a factor for CAD. In conclusion, plasma vinculin concentrations in patients with CAD were not high and were not associated with the presence or severity of CAD.
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