A method of oxygen supply to a low volume tissue bath containing a protein solution

A method that makes it possible to follow the in vitro activity of a ring of the rabbit ear artery submerged in a protein solution for hours is described. The necessary oxygen and carbon dioxide are supplied to the tissue bath through a semipermeable membrane from a pressure chamber. By this procedure, disturbing foam formation is avoided. The tissue bath has a volume of 5 ml. For the rabbit ear artery, it is demonstrated that the response to electrical field stimulation is influenced by the addition of albumin.