Simultaneous carbon dioxide sequestration and utilization for cadaverine production using dual promoters in...

Human carbonic anhydrase II (hCAII) is a rapid-acting zinc-metalloenzyme that catalyzes CO₂ hydration reversibly, with encouraging applications in carbon capture, sequestration, and utilization (CCSU). However, biocatalyst durability is a major challenge. Herein, hCAII is emphasized in 4 different Escherichia coli strains and designated under dual promoters from sigma factor 70 (σ⁷⁰) and heat shock protein (HSP70A) to suppress the usage of inducer and stimulate activity in heat environments. As a result, hCAII under high-efficient dual promoters regulation retained high residual activity in CO₂ biomineralization of 68.8 % after 4 cycles at 40 °C. Moreover, co-expression of CA_C9 with lysine decarboxylase (CadA) simultaneously sequestered CO₂ release up to 95.7 % and increased cadaverine titer from 18.0 to 36.7 g/L by using E. coli MG1655. The remnant biomass from cadaverine synthesis sustained converting CO₂ to 57.9 mg-CaCO₃. Thus, the dual promoters design demonstrated the promising potential for CCSU through simultaneous CO₂ utilization and cadaverine synthesis.